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Home>Product>Primary Antibody

HLA G Recombinant Rabbit mAb

#2147

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Recombinant Antibody
Western blotting analysis using HLA-G antibody (Cat#2147). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HLA-G antibody (Cat#2147, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with HLA-G antibody (Cat#2147, 1:1,000). Nuclei were stained blue with DAPI; HLA-G was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HLA-G
  • Catalog：
    2147
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M

Information

Data

Information
Product Name HLA G Recombinant Rabbit mAb
Aliases HLA-G; Major Histocompatibility Complex, Class I, G; HLA Class I Histocompatibility Antigen, Alpha Chain G; HLA-G Histocompatibility Antigen, Class I, G; MHC Class I Antigen G; B2 Microglobulin; HLA G Antigen; Mutant MHC Class Ib Antigen; Mutant MHC Class I Antigen; MHC Class Ib Antigen; MHC Class I Protein; HLA-6.0; MHC-G; HLAG
Background

Gene Name: HLA-G

NCBI Gene Entry: 3135

UniProt Entry: P17693

Application Information

Molecular Weight: Predicted, 38 kDa; observed, 42 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB6225

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human HLA G
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using HLA-G antibody (Cat#2147). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HLA-G antibody (Cat#2147, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HAP-1 cells with HLA-G antibody (Cat#2147, 1:1,000). Nuclei were stained blue with DAPI; HLA-G was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HLA-G
  • Catalog：
    2147
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M
RELATED PRODUCTS
Western blotting analysis using HLA-G antibody (Cat#2147). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HLA-G antibody (Cat#2147, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with HLA-G antibody (Cat#2147, 1:1,000). Nuclei were stained blue with DAPI; HLA-G was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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