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Home>Product>Primary Antibody

KD-Validated RAD23A Recombinant Rabbit mAb

#62488

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using RAD23A antibody (Cat#62488). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAD23A antibody (Cat#62488, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using RAD23A antibody (Cat#62488). RAD23A expression in wild type (WT) and RAD23A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RAD23A antibody (Cat#62488, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human lymphoma using RAD23A antibody (Cat#62488, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with RAD23A antibody (Cat#62488, 1:1,000). Nuclei were stained blue with DAPI; RAD23A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of Hela cells using RAD23A antibody (Cat#62488, 1:1,000), Top panel: wild-type (WT); Bottom panal: RAD23A shRNA knockdown (KD). Nuclei were stained blue with DAPI; RAD23A was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of RAD23A expression in HepG2 cells using RAD23A antibody (Cat#62488, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, RAD23A.
Validation of RAD23A knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with RAD23A antibody (Cat#62488, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
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  • Gene Symbol：
    RAD23A
  • Catalog：
    62488
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M

Information

Data

Information
Product Name KD-Validated RAD23A Recombinant Rabbit mAb
Aliases RAD23 Homolog A, Nucleotide Excision Repair Protein; HHR23A; UV Excision Repair Protein RAD23 Homolog A; RAD23, Yeast Homolog, A; MGC111083; HR23A; RAD23 (S. Cerevisiae) Homolog A; RAD23 Homolog A (S. Cerevisiae)
Background

Gene Name: RAD23A

NCBI Gene Entry: 5886

UniProt Entry: P54725

Application Information

Molecular Weight: Predicted, 40 kDa; observed, 55 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1080

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human RAD23A
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using RAD23A antibody (Cat#62488). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAD23A antibody (Cat#62488, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using RAD23A antibody (Cat#62488). RAD23A expression in wild type (WT) and RAD23A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RAD23A antibody (Cat#62488, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human lymphoma using RAD23A antibody (Cat#62488, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of HepG2 cells with RAD23A antibody (Cat#62488, 1:1,000). Nuclei were stained blue with DAPI; RAD23A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Immunocytochemical staining of Hela cells using RAD23A antibody (Cat#62488, 1:1,000), Top panel: wild-type (WT); Bottom panal: RAD23A shRNA knockdown (KD). Nuclei were stained blue with DAPI; RAD23A was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of RAD23A expression in HepG2 cells using RAD23A antibody (Cat#62488, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, RAD23A.
  • Validation of RAD23A knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with RAD23A antibody (Cat#62488, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RAD23A
  • Catalog：
    62488
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M
RELATED PRODUCTS
Western blotting analysis using RAD23A antibody (Cat#62488). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAD23A antibody (Cat#62488, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using RAD23A antibody (Cat#62488). RAD23A expression in wild type (WT) and RAD23A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RAD23A antibody (Cat#62488, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human lymphoma using RAD23A antibody (Cat#62488, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with RAD23A antibody (Cat#62488, 1:1,000). Nuclei were stained blue with DAPI; RAD23A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of Hela cells using RAD23A antibody (Cat#62488, 1:1,000), Top panel: wild-type (WT); Bottom panal: RAD23A shRNA knockdown (KD). Nuclei were stained blue with DAPI; RAD23A was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of RAD23A expression in HepG2 cells using RAD23A antibody (Cat#62488, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, RAD23A.
Validation of RAD23A knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with RAD23A antibody (Cat#62488, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
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