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KO-Validated Phospho-TRIM28 (S824) Recombinant Rabbit mAb

#71190

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Recombinant Antibody
Knockout Validated
Western blotting analysis using phospho-TRIM28 (S824) antibody (Cat#71190). Phospho-TRIM28 (S824) expression in wild type (WT) and TRIM28 knockout (KO) HSHC cells with 40 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-TRIM28 (S824) antibody (Cat#71190, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-TRIM28 (S824) expression in HAP-1 cells using Phospho-TRIM28 (S824) antibody (Cat#71190, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-TRIM28 (S824).
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  • Gene Symbol:
    TRIM28
  • Catalog:
    71190
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name KO-Validated Phospho-TRIM28 (S824) Recombinant Rabbit mAb
Aliases TRIM28; Tripartite Motif Containing 28; TIF1B; RNF96; KAP-1; KAP1; Transcription Intermediary Factor 1-Beta; RING Finger Protein 96; TIF1-Beta; PPP1R157; TF1B; Protein Phosphatase 1, Regulatory Subunit 157; RING-Type E3 Ubiquitin Transferase TIF1-Beta; E3 SUMO-Protein Ligase TRIM28; KRAB-Interacting Protein 1; KRAB-Associated Protein 1; Nuclear Corepressor KAP-1; TIF1beta ; KRIP-1; KRAB [Kruppel-Associated Box Domain]-Associated Protein 1; Transcriptional Intermediary Factor 1-Beta; Tripartite Motif-Containing Protein 28; Tripartite Motif-Containing 28; EC 2.3.2.27; TIF1-BETA; TIF1BETA
Background

Gene Name: TRIM28

NCBI Gene Entry: 10155

UniProt Entry: Q13263

Application Information

Molecular Weight: Predicted, 89 kDa; observed, 110 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB2315

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human Phospho-TRIM28 (S824)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using phospho-TRIM28 (S824) antibody (Cat#71190). Phospho-TRIM28 (S824) expression in wild type (WT) and TRIM28 knockout (KO) HSHC cells with 40 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-TRIM28 (S824) antibody (Cat#71190, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of Phospho-TRIM28 (S824) expression in HAP-1 cells using Phospho-TRIM28 (S824) antibody (Cat#71190, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-TRIM28 (S824).
Western blotting analysis using phospho-TRIM28 (S824) antibody (Cat#71190). Phospho-TRIM28 (S824) expression in wild type (WT) and TRIM28 knockout (KO) HSHC cells with 40 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-TRIM28 (S824) antibody (Cat#71190, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-TRIM28 (S824) expression in HAP-1 cells using Phospho-TRIM28 (S824) antibody (Cat#71190, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-TRIM28 (S824).
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