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PDIA3 Mouse mAb

#6607

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Western blotting analysis using PDIA3 antibody (Cat#6607). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PDIA3 antibody (Cat#6607, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PDIA3 expression in HAP-1 cells using PDIA3 antibody (Cat#6607, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PDIA3.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    PDIA3
  • Catalog:
    6607
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name PDIA3 Mouse mAb
Aliases PDIA3; Protein Disulfide Isomerase Family A Member 3; ERp57; ERp60; P58; HsT17083; PI-PLC; ERp61; GRP57; GRP58; Endoplasmic Reticulum Resident Protein 57; Endoplasmic Reticulum Resident Protein 60; Protein Disulfide Isomerase-Associated 3; Glucose Regulated Protein, 58kDa; 58 KDa Glucose-Regulated Protein; Protein Disulfide-Isomerase A3; 58 KDa Microsomal Protein; Disulfide Isomerase ER-60; ER Protein 57; ER Protein 60; EC 5.3.4.1; Epididymis Secretory Sperm Binding Protein Li 93n; Protein Disulfide Isomerase Family A, Member 3; Epididymis Secretory Protein Li 269; Endoplasmic Reticulum P58; Phospholipase C-Alpha; HEL-S-269; HEL-S-93n; ERP57; ERP60; ER60
Background

Gene Name: PDIA3

NCBI Gene Entry: 2923

UniProt Entry: P30101

Application Information

Molecular Weight: Predicted, 57 kDa; observed, 51 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB110

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human PDIA3
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using PDIA3 antibody (Cat#6607). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PDIA3 antibody (Cat#6607, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of PDIA3 expression in HAP-1 cells using PDIA3 antibody (Cat#6607, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PDIA3.
Western blotting analysis using PDIA3 antibody (Cat#6607). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PDIA3 antibody (Cat#6607, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PDIA3 expression in HAP-1 cells using PDIA3 antibody (Cat#6607, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PDIA3.
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