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PSMA3 Mouse mAb

#6315

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Western blotting analysis using PSMA3 antibody (Cat#6315). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PSMA3 antibody (Cat#6315, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PSMA3 expression in HepG2 cells using PSMA3 antibody (Cat#6315,1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PSMA3.
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Unit price:
$69.00
Quantity:
1
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  • Gene Symbol:
    PSMA3
  • Catalog:
    6315
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name PSMA3 Mouse mAb
Aliases PSMA3; Proteasome 20S Subunit Alpha 3; HC8; Proteasome (Prosome, Macropain) Subunit, Alpha Type, 3; Multicatalytic Endopeptidase Complex Subunit C8; Proteasome Subunit Alpha Type-3; Proteasome Subunit Alpha 3; Proteasome Subunit Alpha-7; Proteasome Component C8; Macropain Subunit C8; Alpha-7; Testicular Secretory Protein Li 43; Proteasome Subunit Alpha7; Proteasome Subunit Α7; Proteasome Subunit C8; EC 3.4.25.1; PSC3; PSC8
Background

Gene Name: PSMA3

NCBI Gene Entry: 5684

UniProt Entry: P25788

Application Information

Molecular Weight: Predicted, 28 kDa; observed, 28 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB8535

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human PSMA3
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using PSMA3 antibody (Cat#6315). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PSMA3 antibody (Cat#6315, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of PSMA3 expression in HepG2 cells using PSMA3 antibody (Cat#6315,1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PSMA3.
Western blotting analysis using PSMA3 antibody (Cat#6315). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PSMA3 antibody (Cat#6315, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PSMA3 expression in HepG2 cells using PSMA3 antibody (Cat#6315,1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PSMA3.
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