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KO-Validated MS4A14 Recombinant Rabbit mAb

#71215

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Recombinant Antibody
Knockout Validated
Western blotting analysis using membrane spanning 4-domains A14 antibody (Cat#71215). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with membrane spanning 4-domains A14 antibody (Cat#71215, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using membrane spanning 4-domains A14 antibody (Cat#71215). Membrane spanning 4-domains A14 expression in wild type (WT) and membrane spanning 4-domains A14 (MS4A14) knockout (KO) HSHC cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with membrane spanning 4-domains A14 antibody (Cat#71215, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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  • Gene Symbol:
    MS4A14
  • Catalog:
    71215
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KO-Validated MS4A14 Recombinant Rabbit mAb
Aliases MS4A14; Membrane Spanning 4-Domains A14; NYD-SP21; MS4A16; Membrane-Spanning 4-Domains, Subfamily A, Member 16; Membrane-Spanning 4-Domains Subfamily A Member 14; Testis Development Protein NYD-SP21; DKFZp434H092; FLJ32856; Membrane-Spanning 4-Domains, Subfamily A, Member 14; Testes Development-Related NYD-SP21; MS4A13 Protein; MS4A13
Background

Gene Name: MS4A14

NCBI Gene Entry: 84689

UniProt Entry: Q96JA4

Application Information

Molecular Weight: Predicted, 77 kDa; observed, 70 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB3010

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human MS4A14
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using membrane spanning 4-domains A14 antibody (Cat#71215). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with membrane spanning 4-domains A14 antibody (Cat#71215, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using membrane spanning 4-domains A14 antibody (Cat#71215). Membrane spanning 4-domains A14 expression in wild type (WT) and membrane spanning 4-domains A14 (MS4A14) knockout (KO) HSHC cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with membrane spanning 4-domains A14 antibody (Cat#71215, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using membrane spanning 4-domains A14 antibody (Cat#71215). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with membrane spanning 4-domains A14 antibody (Cat#71215, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using membrane spanning 4-domains A14 antibody (Cat#71215). Membrane spanning 4-domains A14 expression in wild type (WT) and membrane spanning 4-domains A14 (MS4A14) knockout (KO) HSHC cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with membrane spanning 4-domains A14 antibody (Cat#71215, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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