KO-Validated CAMK1 Recombinant Rabbit mAb - Product

KO-Validated CAMK1 Recombinant Rabbit mAb

#71153

Click to copy product information

Return to List

Recombinant Antibody
Knockdown Validated

Western blotting analysis using CAMK1 antibody (Cat#71153). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CAMK1 antibody (Cat#71153, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using CAMK1 antibody (Cat#71153). CAMK1 expression in wild-type (WT) and CAMK1 knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CAMK1 antibody (Cat#71153, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Flow cytometric analysis of CAMK1 expression in HeLa cells using CAMK1 antibody (Cat# 71153, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CAMK1.

Please select the specifications of the product.

Specification：

Unit price：

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

CAMK1
Catalog：

71153
Application：

WB, FCM
Species Reactivity：

H, M, R

Information

Data

Information

Product Name	KO-Validated CAMK1 Recombinant Rabbit mAb
Aliases	CAMK1; Calcium/Calmodulin Dependent Protein Kinase I 2; CaMKI-Alpha; CaMKI ; Calcium/Calmodulin-Dependent Protein Kinase Type 1; CaM Kinase I Alpha; EC 2.7.11.17; CaM-KI; CaM Kinase I; EC 2.7.11
Background	Gene Name: CAMK1 NCBI Gene Entry: 8536 UniProt Entry: Q14012
Application Information	Molecular Weight: Predicted, 41 kDa; observed, 41 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB570 Species Reactivity: Human,mouse,rat Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human CaMKI
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:100-1:1,000
Note	This product is for research use only.

Data

Western blotting analysis using CAMK1 antibody (Cat#71153). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CAMK1 antibody (Cat#71153, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using CAMK1 antibody (Cat#71153). CAMK1 expression in wild-type (WT) and CAMK1 knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CAMK1 antibody (Cat#71153, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CAMK1 expression in HeLa cells using CAMK1 antibody (Cat# 71153, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CAMK1.

Please select the specifications of the product.

Specification：

Unit price：

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

CAMK1
Catalog：

71153
Application：

WB, FCM
Species Reactivity：

H, M, R

Datasheet

Home

About

Standards

Product

Custom Services

Signal Pathways

Technical Support

News