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KD-Validated NDUFB10 Recombinant Rabbit mAb

#66906

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using NDUFB10 antibody (Cat#66906). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFB10 antibody (Cat#66906, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using NDUFB10 antibody (Cat#66906). NDUFB10 expression in wild type (WT) and NDUFB10 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NDUFB10 antibody (Cat#66906, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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  • Gene Symbol:
    NDUFB10
  • Catalog:
    66906
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KD-Validated NDUFB10 Recombinant Rabbit mAb
Aliases NDUFB10; NADH:Ubiquinone Oxidoreductase Subunit B10; PDSW; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 10, 22kDa; NADH Dehydrogenase [Ubiquinone] 1 Beta Subcomplex Subunit 10; NADH-Ubiquinone Oxidoreductase PDSW Subunit; Complex I PDSW Subunit; Complex I-PDSW; CI-PDSW; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 10 (22kD, PDSW); NADH Ubiquinone Oxidoreductase PDSW Subunit (RH 16p13.3); MC1DN35
Background

Gene Name: NDUFB10

NCBI Gene Entry: 4716

UniProt Entry: O96000

Application Information

Molecular Weight: Predicted, 21 kDa; observed, 21 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB1280

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen Recombinant protein of human NDUFB10
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using NDUFB10 antibody (Cat#66906). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFB10 antibody (Cat#66906, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using NDUFB10 antibody (Cat#66906). NDUFB10 expression in wild type (WT) and NDUFB10 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NDUFB10 antibody (Cat#66906, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using NDUFB10 antibody (Cat#66906). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFB10 antibody (Cat#66906, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using NDUFB10 antibody (Cat#66906). NDUFB10 expression in wild type (WT) and NDUFB10 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NDUFB10 antibody (Cat#66906, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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