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L1 Cell Adhesion Molecule Recombinant Rabbit mAb

#3296

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Recombinant Antibody
Western blotting analysis using L1 cell adhesion molecule antibody (Cat#3296). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with L1 cell adhesion molecule antibody (Cat#3296, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of L1 cell adhesion molecule expression in HepG2 cells using L1 cell adhesion molecule antibody (Cat#3296, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, L1 cell adhesion molecule.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    L1CAM
  • Catalog:
    3296
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name L1 Cell Adhesion Molecule Recombinant Rabbit mAb
Aliases L1CAM; L1 Cell Adhesion Molecule; NCAM-L1; CAML1; Neural Cell Adhesion Molecule L1; CD171; MIC5; Antigen Identified By Monoclonal Antibody R1; N-CAM-L1; HSAS1; HSAS; MASA; SPG1; S10; CD171 Antigen; N-CAML1; HYCX
Background

Gene Name: L1CAM

NCBI Gene Entry: 3897

UniProt Entry: P32004

Application Information

Molecular Weight: Predicted, 140 kDa; observed, 250 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4935

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human L1CAM
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using L1 cell adhesion molecule antibody (Cat#3296). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with L1 cell adhesion molecule antibody (Cat#3296, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of L1 cell adhesion molecule expression in HepG2 cells using L1 cell adhesion molecule antibody (Cat#3296, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, L1 cell adhesion molecule.
Western blotting analysis using L1 cell adhesion molecule antibody (Cat#3296). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with L1 cell adhesion molecule antibody (Cat#3296, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of L1 cell adhesion molecule expression in HepG2 cells using L1 cell adhesion molecule antibody (Cat#3296, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, L1 cell adhesion molecule.
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