Immunocytochemical staining shows negative staining in paraffin-embedded human colon (negative tissue). GCDFP-15 antibody (Cat# H1171) was used at 1:250 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human lung squamous cell cancer (negative tissue). GCDFP-15 antibody (Cat# H1171) was used at 1:250 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human breast cancer. GCDFP-15 antibody (Cat# H1171) was used at 1:250 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.