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Home>Product>Primary Antibody

EIF3E Recombinant Rabbit mAb

#2083

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Recombinant Antibody
Western blotting analysis using EIF3E antibody (Cat#2083). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EIF3E antibody (Cat#2083, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells with EIF3E antibody (Cat#2083, 1:1,000). Nuclei were stained blue with DAPI; EIF3E was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of EIF3E expression in HeLa cells using EIF3E antibody (Cat#2083, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, EIF3E.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    EIF3E
  • Catalog：
    2083
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name EIF3E Recombinant Rabbit mAb
Aliases EIF3E; Eukaryotic Translation Initiation Factor 3 Subunit E; EIF3-P48; EIF3S6; INT6; Eukaryotic Translation Initiation Factor 3, Subunit 6 48kDa; Eukaryotic Translation Initiation Factor 3 Subunit 6; Viral Integration Site Protein INT-6 Homolog; EIF-3 P48; Eukaryotic Translation Initiation Factor 3, Subunit 6 (48kD); Murine Mammary Tumor Integration Site 6 (Oncogene Homolog); Eukaryotic Translation Initiation Factor 3, Subunit E; Mammary Tumor-Associated Protein INT6; EIF3-P46; EIF3e
Background

Gene Name: EIF3E

NCBI Gene Entry: 3646

UniProt Entry: P60228

Application Information

Molecular Weight: Predicted, 52 kDa; observed, 45 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB5915

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human eIF3e
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using EIF3E antibody (Cat#2083). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EIF3E antibody (Cat#2083, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HeLa cells with EIF3E antibody (Cat#2083, 1:1,000). Nuclei were stained blue with DAPI; EIF3E was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of EIF3E expression in HeLa cells using EIF3E antibody (Cat#2083, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, EIF3E.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    EIF3E
  • Catalog：
    2083
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using EIF3E antibody (Cat#2083). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EIF3E antibody (Cat#2083, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells with EIF3E antibody (Cat#2083, 1:1,000). Nuclei were stained blue with DAPI; EIF3E was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of EIF3E expression in HeLa cells using EIF3E antibody (Cat#2083, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, EIF3E.
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