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IL9 Recombinant Rabbit mAb

#2200

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Recombinant Antibody
Western blotting analysis using IL9 antibody (Cat#2200). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IL9 antibody (Cat#2200, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with IL9 antibody (Cat#2200, 1:1,000). Nuclei were stained blue with DAPI; IL9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    IL9
  • Catalog:
    2200
  • Application:
    WB, ICC
  • Species Reactivity:
    H
Information
Product Name IL9 Recombinant Rabbit mAb
Aliases IL9; Interleukin 9; IL-9; T-Cell Growth Factor P40; HP40; P40; Homolog Of Mouse T Cell And Mast Cell Growth Factor 40; P40 T-Cell And Mast Cell Growth Factor; Interleukin-9; P40 Cytokine; Cytokine P40
Background

Gene Name: IL9

NCBI Gene Entry: 3578

UniProt Entry: P15248

Application Information

Molecular Weight: Predicted, 16 kDa; observed, 16 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB6475

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human IL9
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using IL9 antibody (Cat#2200). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IL9 antibody (Cat#2200, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HT-1080 cells with IL9 antibody (Cat#2200, 1:1,000). Nuclei were stained blue with DAPI; IL9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Western blotting analysis using IL9 antibody (Cat#2200). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IL9 antibody (Cat#2200, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with IL9 antibody (Cat#2200, 1:1,000). Nuclei were stained blue with DAPI; IL9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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