• Primary Antibody
  • Secondary Antibodies
  • Lentiviral Particles
  • Stable Cell Lines
  • Lysates
  • Reagents
  • Other Products

KD-Validated UBA1 Recombinant Rabbit mAb

#62001

Click to copy product information
Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using UBA1 antibody (Cat#62001). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBA1 antibody (Cat#62001, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using UBA1 antibody (Cat#62001). UBA1 expression in wild-type (WT) and UBA1 shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with UBA1 antibody (Cat#62001, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    UBA1
  • Catalog:
    62001
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KD-Validated UBA1 Recombinant Rabbit mAb
Aliases UBA1; Ubiquitin Like Modifier Activating Enzyme 1; CFAP124; UBE1X; POC20; A1S9T; UBE1; Ubiquitin-Like Modifier-Activating Enzyme 1; Ubiquitin-Activating Enzyme E1; GXP1; Ubiquitin-Activating Enzyme E1 (A1S9T And BN75 Temperature Sensitivity Complementing); UBA1, Ubiquitin-Activating Enzyme E1 Homolog (Yeast); A1S9T And BN75 Temperature Sensitivity Complementing; POC20 Centriolar Protein Homolog (Chlamydomonas); UBA1, Ubiquitin-Activating Enzyme E1 Homolog A; Ubiquitin-Like Modifier Activating Enzyme 1; Testicular Secretory Protein Li 63; POC20 Centriolar Protein Homolog; Protein A1S9; EC 6.2.1.45; AMCX1; SMAX2; UBA1A; VEXAS; A1S9; A1ST
Background

Gene Name: UBA1

NCBI Gene Entry: 7317

UniProt Entry: P22314

Application Information

Molecular Weight: Predicted, 118 kDa; observed, 118 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB7810

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human UBA1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using UBA1 antibody (Cat#62001). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBA1 antibody (Cat#62001, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using UBA1 antibody (Cat#62001). UBA1 expression in wild-type (WT) and UBA1 shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with UBA1 antibody (Cat#62001, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using UBA1 antibody (Cat#62001). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBA1 antibody (Cat#62001, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using UBA1 antibody (Cat#62001). UBA1 expression in wild-type (WT) and UBA1 shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with UBA1 antibody (Cat#62001, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Phone Number

    +1 (301) 545-2395

  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

微信公众号

Copyright © 2025 Hefei GenuIN Biotech Co., Ltd.

All Rights Reserved.