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Home>Product>Primary Antibody

Keratin 10 Recombinant Rabbit mAb

#4157

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Recombinant Antibody
Western blotting analysis using keratin 10 antibody (Cat#4157). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with keratin 10 antibody (Cat#4157, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human skin tissue using keratin 10 antibody (Cat#4157, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    KRT10
  • Catalog：
    4157
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name Keratin 10 Recombinant Rabbit mAb
Aliases KRT10; Keratin 10; K10; CK10; KPP; Keratin, Type I Cytoskeletal 10; Keratin 10, Type I; Cytokeratin 10; CK-10; Keratosis Palmaris Et Plantaris; Epidermolytic Hyperkeratosis; Cytokeratin-10; Keratin-10; BCIE; EHK2; BIE; EHK; IHL
Background

Gene Name: KRT10

NCBI Gene Entry: 3858

UniProt Entry: P13645

Application Information

Molecular Weight: Predicted, 59 kDa; observed, 60 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB9840

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human Cytokeratin 10
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western blotting (WB): 1:1,000-1:5,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using keratin 10 antibody (Cat#4157). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with keratin 10 antibody (Cat#4157, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunohistochemistry was performed on paraffin-embedded human skin tissue using keratin 10 antibody (Cat#4157, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    KRT10
  • Catalog：
    4157
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using keratin 10 antibody (Cat#4157). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with keratin 10 antibody (Cat#4157, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human skin tissue using keratin 10 antibody (Cat#4157, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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