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Home>Product>Primary Antibody

Alkaline Phosphatase Recombinant Rabbit mAb

#9464

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Recombinant Antibody
Western blotting analysis using Alkaline phosphatase antibody (Cat#9464). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Alkaline phosphatase antibody (Cat#9464, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells with Alkaline phosphatase antibody (Cat#9464, 1:1,000). Nuclei were stained blue with DAPI; Alkaline phosphatase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Alkaline phosphatase expression in HeLa cells using Alkaline phosphatase antibody (Cat#9464, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Alkaline phosphatase.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ALPL
  • Catalog：
    9464
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Alkaline Phosphatase Recombinant Rabbit mAb
Aliases Alkaline Phosphatase, Biomineralization Associated; TNSALP; TNALP; TNAP; Alkaline Phosphatase, Tissue-Nonspecific Isozyme; Alkaline Phosphatase Liver/Bone/Kidney Isozyme; Tissue Non-Specific Alkaline Phosphatase; Alkaline Phosphatase, Liver/Bone/Kidney; Phosphocreatine Phosphatase; Phosphoamidase; EC 3.1.3.1; AP-TNAP; TNS-ALP; HOPS; Liver/Bone/Kidney-Type Alkaline Phosphatase; Tissue-Nonspecific ALP; EC 3.9.1.1; APTNAP; HPPA; HPPC; HPPI; HPPO
Background

Gene Name: ALPL

NCBI Gene Entry: 249

UniProt Entry: P05186

Application Information

Molecular Weight: Predicted, 57 kDa; observed, 70 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1765

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Alkaline Phosphatase
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Alkaline phosphatase antibody (Cat#9464). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Alkaline phosphatase antibody (Cat#9464, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HeLa cells with Alkaline phosphatase antibody (Cat#9464, 1:1,000). Nuclei were stained blue with DAPI; Alkaline phosphatase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Alkaline phosphatase expression in HeLa cells using Alkaline phosphatase antibody (Cat#9464, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Alkaline phosphatase.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ALPL
  • Catalog：
    9464
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Alkaline phosphatase antibody (Cat#9464). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Alkaline phosphatase antibody (Cat#9464, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells with Alkaline phosphatase antibody (Cat#9464, 1:1,000). Nuclei were stained blue with DAPI; Alkaline phosphatase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Alkaline phosphatase expression in HeLa cells using Alkaline phosphatase antibody (Cat#9464, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Alkaline phosphatase.
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