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Home>Product>Primary Antibody

IL-33 Recombinant Rabbit mAb

#2206

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Recombinant Antibody
Western blotting analysis using IL33 antibody (Cat#2206). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IL33 antibody (Cat#2206, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with IL33 antibody (Cat#2206, 1:1,000). Nuclei were stained blue with DAPI; IL33 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    IL33
  • Catalog：
    2206
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name IL-33 Recombinant Rabbit mAb
Aliases IL33; Interleukin 33; NF-HEV; IL1F11; C9orf26; DVS27; Nuclear Factor From High Endothelial Venules; Nuclear Factor For High Endothelial Venules; Interleukin-1 Family Member 11; DVS27-Related Protein; Interleukin-33; DKFZp586H0523; Chromosome 9 Open Reading Frame 26 (NF-HEV); Interleukin-1 Family, Member 11; IL-1F11; NFEHEV; NFHEV; IL-33
Background

Gene Name: IL33

NCBI Gene Entry: 90865

UniProt Entry: O95760

Application Information

Molecular Weight: Predicted, 31 kDa; observed, 33 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB6505

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from mouse IL-33
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using IL33 antibody (Cat#2206). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IL33 antibody (Cat#2206, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with IL33 antibody (Cat#2206, 1:1,000). Nuclei were stained blue with DAPI; IL33 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    IL33
  • Catalog：
    2206
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using IL33 antibody (Cat#2206). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IL33 antibody (Cat#2206, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with IL33 antibody (Cat#2206, 1:1,000). Nuclei were stained blue with DAPI; IL33 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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