Cart

Home

About

Standards

Product

Custom Services

Signal Pathways

Technical Support

News

Sign In/Register

Cart
  • Primary Antibody
  • Secondary Antibodies
  • Lentiviral Particles
  • Stable Cell Lines
  • Lysates
  • Reagents
  • Other Products
Home>Product>Primary Antibody

P4HTM Recombinant Rabbit mAb

#2278

Click to copy product information

Return to List

Recombinant Antibody
Western blotting analysis using P4HTM antibody (Cat#2278). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with P4HTM antibody (Cat#2278, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with P4HTM antibody (Cat#2278, 1:1,000). Nuclei were stained blue with DAPI; P4HTM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of P4HTM expression in HepG2 cells using P4HTM antibody (Cat#2278, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, P4HTM.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    P4HTM
  • Catalog：
    2278
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name P4HTM Recombinant Rabbit mAb
Aliases Prolyl 4-Hydroxylase, Transmembrane; P4H-TM; PH4; HIFPH4; EGLN4; PHD4; PH-4; Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum); Hypoxia-Inducible Factor Prolyl Hydroxylase 4; Prolyl Hydroxlase Domain-Containing 4; Transmembrane Prolyl 4-Hydroxylase; HIF-Prolyl Hydroxylase 4; FLJ20262; HIF-PH4; HPH-4; Hypoxia Inducible Factor Prolyl 4 Hydroxylase; Hypoxia-Inducible Factor Prolyl 4-Hydroxylase; P4H With Transmembrane Domain; Proline 4-Hydroxylase; EC 1.14.11.29; HIDEA
Background

Gene Name: P4HTM

NCBI Gene Entry: 54681

UniProt Entry: Q9NXG6

Application Information

Molecular Weight: Predicted, 57 kDa; observed, 47 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB100

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human HIF Prolyl Hydroxylases
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using P4HTM antibody (Cat#2278). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with P4HTM antibody (Cat#2278, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with P4HTM antibody (Cat#2278, 1:1,000). Nuclei were stained blue with DAPI; P4HTM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of P4HTM expression in HepG2 cells using P4HTM antibody (Cat#2278, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, P4HTM.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    P4HTM
  • Catalog：
    2278
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using P4HTM antibody (Cat#2278). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with P4HTM antibody (Cat#2278, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with P4HTM antibody (Cat#2278, 1:1,000). Nuclei were stained blue with DAPI; P4HTM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of P4HTM expression in HepG2 cells using P4HTM antibody (Cat#2278, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, P4HTM.
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$
View cart Empty cart
  • Phone Number

    +1 (301) 545-2395

  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

微信公众号

Copyright © 2025 Hefei GenuIN Biotech Co., Ltd.

All Rights Reserved.