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Tumor Protein P53 Binding Protein 2 Recombinant Rabbit mAb

#2652

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Recombinant Antibody
Western blotting analysis using TP53BP2 antibody (Cat#2652). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TP53BP2 antibody (Cat#2652, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with TP53BP2 antibody (Cat#2652, 1:1,000). Nuclei were stained blue with DAPI;TP53BP2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of TP53BP2 expression in HepG2 cells using TP53BP2 antibody (Cat#2652, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TP53BP2.
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Unit price:
$69.00
Quantity:
1
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  • Gene Symbol:
    TP53BP2
  • Catalog:
    2652
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name Tumor Protein P53 Binding Protein 2 Recombinant Rabbit mAb
Aliases TP53BP2; Tumor Protein P53 Binding Protein 2; ASPP2; 53BP2; PPP1R13A; Apoptosis-Stimulating Of P53 Protein 2; Tumor Suppressor P53-Binding Protein 2; Renal Carcinoma Antigen NY-REN-51; P53BP2; BBP; Apoptosis-Stimulating Protein Of P53, 2; Tumor Protein P53-Binding Protein, 2; P53-Binding Protein 2; BCL2-Binding Protein; Bcl2-Binding Protein; Bbp
Background

Gene Name: TP53BP2

NCBI Gene Entry: 7159

UniProt Entry: Q13625

Application Information

Molecular Weight: Predicted, 126 kDa; observed, 147 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2145

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human ASPP2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using TP53BP2 antibody (Cat#2652). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TP53BP2 antibody (Cat#2652, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with TP53BP2 antibody (Cat#2652, 1:1,000). Nuclei were stained blue with DAPI;TP53BP2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of TP53BP2 expression in HepG2 cells using TP53BP2 antibody (Cat#2652, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TP53BP2.
Western blotting analysis using TP53BP2 antibody (Cat#2652). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TP53BP2 antibody (Cat#2652, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with TP53BP2 antibody (Cat#2652, 1:1,000). Nuclei were stained blue with DAPI;TP53BP2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of TP53BP2 expression in HepG2 cells using TP53BP2 antibody (Cat#2652, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TP53BP2.
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