Western blotting analysis using asparagine synthetase antibody (Cat#71271). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with asparagine synthetase antibody (Cat#71271, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using asparagine synthetase antibody (Cat#71271). Asparagine synthetase expression in wild type (WT) and asparagine synthetase (ASNS) knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with asparagine synthetase antibody (Cat#71271, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Asparagine synthetase expression in HAP-1 cells using Asparagine synthetase antibody (Cat#71271, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Asparagine synthetase.
