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Home>Product>Primary Antibody

ABHD5 Recombinant Rabbit mAb

#4620

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Recombinant Antibody
Western blotting analysis using ABHD5 antibody (Cat#4620). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ABHD5 antibody (Cat#4620, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with ABHD5 antibody (Cat#4620, 1:1,000). Nuclei were stained blue with DAPI; ABHD5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ABHD5 expression in C2C12 cells using ABHD5 antibody (Cat#4620, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ABHD5.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ABHD5
  • Catalog：
    4620
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name ABHD5 Recombinant Rabbit mAb
Aliases ABHD5; Antibodyhydrolase Domain Containing 5, Lysophosphatidic Acid Acyltransferase; NCIE2; 1-Acylglycerol-3-Phosphate O-Acyltransferase ABHD5; Antibodyhydrolase Domain-Containing Protein 5; Lipid Droplet-Binding Protein CGI-58; EC 2.3.1.51; CGI-58; Antibodyhydrolase Domain Containing 5; CGI58; IECN2
Background

Gene Name: ABHD5

NCBI Gene Entry: 51099

UniProt Entry: Q8WTS1

Application Information

Molecular Weight: Predicted, 39 kDa; observed, 45 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB12110

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human ABHD5
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ABHD5 antibody (Cat#4620). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ABHD5 antibody (Cat#4620, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of C2C12 cells with ABHD5 antibody (Cat#4620, 1:1,000). Nuclei were stained blue with DAPI; ABHD5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of ABHD5 expression in C2C12 cells using ABHD5 antibody (Cat#4620, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ABHD5.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ABHD5
  • Catalog：
    4620
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using ABHD5 antibody (Cat#4620). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ABHD5 antibody (Cat#4620, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with ABHD5 antibody (Cat#4620, 1:1,000). Nuclei were stained blue with DAPI; ABHD5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ABHD5 expression in C2C12 cells using ABHD5 antibody (Cat#4620, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ABHD5.
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