Western blotting analysis using anti-CREB1 antibody (Cat#R0018). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CREB1 antibody (Cat#R0018, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with anti-CREB1 antibody (Cat#R0018, 1:1,000) . Nuclei were stained blue with DAPI; CREB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen
A synthesized peptide derived from human CREB
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 40% glycerol, 0.05% BSA and 0.03% Proclin 300.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:400-1:2,000 Immunocytochemistry (ICC): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using anti-CREB1 antibody (Cat#R0018). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CREB1 antibody (Cat#R0018, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with anti-CREB1 antibody (Cat#R0018, 1:1,000) . Nuclei were stained blue with DAPI; CREB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-CREB1 antibody (Cat#R0018). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CREB1 antibody (Cat#R0018, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with anti-CREB1 antibody (Cat#R0018, 1:1,000) . Nuclei were stained blue with DAPI; CREB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001731s ]
SELECT * FROM `goods` WHERE `code` = 'R0018' LIMIT 1 [ RunTime:0.001392s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.001337s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000901s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000740s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 349 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001390s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.001019s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 349 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001161s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000929s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000636s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.129771s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5759,8574,9628,6559,5574,9505,95,8333,9183,9379) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001032s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.002293s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.001722s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.003757s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000620s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000773s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000844s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.002606s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000210s ]
