Western blotting analysis using charged multivesicular body protein 5 antibody (Cat#63819). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with charged multivesicular body protein 5 antibody (Cat#63819, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using charged multivesicular body protein 5 antibody (Cat#63819). Charged multivesicular body protein 5 expression in wild-type (WT) and charged multivesicular body protein 5 (CHMP5) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with charged multivesicular body protein 5 antibody (Cat#63819, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Charged multivesicular body protein 5 expression in HepG2 cells using Charged multivesicular body protein 5 antibody (Cat#63819, 1:1,000). Green, isotype control; red, Charged multivesicular body protein 5.
CHMP5; Charged Multivesicular Body Protein 5; Vps60; HSPC177; C9orf83; SNF7DC2; CGI-34; Vacuolar Protein Sorting-Associated Protein 60; SNF7 Domain-Containing Protein; Chromatin-Modifying Protein 5; HVps60; Chromosome 9 Open Reading Frame 83; Apoptosis-Related Protein PNAS-2; Chromatin Modifying Protein 5; SNF7 Domain Containing; PNAS-2
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human CHMP5
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:200-1:1,000 Flow Cytometry (FCM): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using charged multivesicular body protein 5 antibody (Cat#63819). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with charged multivesicular body protein 5 antibody (Cat#63819, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using charged multivesicular body protein 5 antibody (Cat#63819). Charged multivesicular body protein 5 expression in wild-type (WT) and charged multivesicular body protein 5 (CHMP5) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with charged multivesicular body protein 5 antibody (Cat#63819, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Charged multivesicular body protein 5 expression in HepG2 cells using Charged multivesicular body protein 5 antibody (Cat#63819, 1:1,000). Green, isotype control; red, Charged multivesicular body protein 5.
Western blotting analysis using charged multivesicular body protein 5 antibody (Cat#63819). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with charged multivesicular body protein 5 antibody (Cat#63819, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using charged multivesicular body protein 5 antibody (Cat#63819). Charged multivesicular body protein 5 expression in wild-type (WT) and charged multivesicular body protein 5 (CHMP5) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with charged multivesicular body protein 5 antibody (Cat#63819, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Charged multivesicular body protein 5 expression in HepG2 cells using Charged multivesicular body protein 5 antibody (Cat#63819, 1:1,000). Green, isotype control; red, Charged multivesicular body protein 5.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000757s ]
SELECT * FROM `goods` WHERE `code` = '63819' LIMIT 1 [ RunTime:0.002464s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000501s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001653s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.002529s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 8333 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000491s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.001957s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 8333 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000288s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000364s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000264s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.072981s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (6481,7025,7125,155,101,4357,6842,8685,8,4938) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002409s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000731s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000353s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000683s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000353s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000490s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000558s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000362s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000313s ]
