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Home>Product>Primary Antibody

KD-Validated Epidermal Growth Factor Receptor Rabbit pAb

#62827

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shRNA Knockdown Validated
Western blotting analysis using Epidermal growth factor receptor antibody (Cat#62827). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Epidermal growth factor receptor antibody (Cat#62827, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Epidermal growth factor receptor antibody (Cat#62827). Epidermal growth factor receptor expression in wild type (WT) and Epidermal growth factor receptor shRNA knockdown (KD) HeLa cells with 10 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Epidermal growth factor receptor antibody (Cat#62827, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    EGFR
  • Catalog：
    62827
  • Application：
    WB
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated Epidermal Growth Factor Receptor Rabbit pAb
Aliases EGFR; Epidermal Growth Factor Receptor; ERBB1; ERRP; ERBB; Receptor Tyrosine-Protein Kinase ErbB-1; Erb-B2 Receptor Tyrosine Kinase 1; Proto-Oncogene C-ErbB-1; EC 2.7.10.1; HER1; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian); Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog; Epidermal Growth Factor Receptor Tyrosine Kinase Domain; Cell Proliferation-Inducing Protein 61; Cell Growth Inhibiting Protein 40; EGFR VIII; EC 2.7.10; NISBD2; PIG61; MENA
Background

Gene Name: EGFR

NCBI Gene Entry: 1956

UniProt Entry: P00533

Application Information

Molecular Weight: Predicted, 134 kDa; observed, 175 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human

Applications Tested: Western blotting (WB)
Immunogen A synthesized peptide derived from human EGFR
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,500-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using Epidermal growth factor receptor antibody (Cat#62827). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Epidermal growth factor receptor antibody (Cat#62827, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using Epidermal growth factor receptor antibody (Cat#62827). Epidermal growth factor receptor expression in wild type (WT) and Epidermal growth factor receptor shRNA knockdown (KD) HeLa cells with 10 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Epidermal growth factor receptor antibody (Cat#62827, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    EGFR
  • Catalog：
    62827
  • Application：
    WB
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using Epidermal growth factor receptor antibody (Cat#62827). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Epidermal growth factor receptor antibody (Cat#62827, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Epidermal growth factor receptor antibody (Cat#62827). Epidermal growth factor receptor expression in wild type (WT) and Epidermal growth factor receptor shRNA knockdown (KD) HeLa cells with 10 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Epidermal growth factor receptor antibody (Cat#62827, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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