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Home>Product>Primary Antibody

Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Recombinant Rabbit mAb

#2355

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Recombinant Antibody
Western blotting analysis using Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) antibody (Cat#2355). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) antibody (Cat#2355, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of H9C2 cells with Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) antibody (Cat#2355, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) expression in H9c2 cells using Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) antibody (Cat#2355, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PAK4/PAK5/PAK6
  • Catalog：
    2355
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Recombinant Rabbit mAb
Aliases P21 (RAC1) Activated Kinase 4; P21 Protein (Cdc42/Rac)-Activated Kinase 4; Serine/Threonine-Protein Kinase PAK 4; P21(CDKN1A)-Activated Kinase 4; EC 2.7.11.1; Protein Kinase Related To S. Cerevisiae STE20, Effector For Cdc42Hs; P21-Activated Kinase 4; EC 2.7.11; KIAA1142; PAK-4; P21 (RAC1) Activated Kinase 5; KIAA1264; PAK7; P21 Protein (Cdc42/Rac)-Activated Kinase 7; Serine/Threonine-Protein Kinase PAK 5; P21(CDKN1A)-Activated Kinase 7; P21 (RAC1) Activated Kinase 7; P21-Activated Kinase 5; P21-Activated Kinase 7; EC 2.7.11.1; PAK-5; PAK-7; Serine/Threonine-Protein Kinase PAK 7; Serine/Threonine-Protein Kinase PAK7; P21CDKN1A-Activated Kinase 7; Protein Kinase PAK5; EC 2.7.11; P21 (RAC1) Activated Kinase 6; PAK5; P21 Protein (Cdc42/Rac)-Activated Kinase 6; Serine/Threonine-Protein Kinase PAK 6; P21(CDKN1A)-Activated Kinase 6; EC 2.7.11.1; P21-Activated Kinase 6; EC 2.7.11; PAK-5; PAK-6
Background

Gene Name: PAK4/PAK5/PAK6

NCBI Gene Entry: 10298 , 57144 , 56924

UniProt Entry: O96013 , Q9P286 , Q9NQU5

Application Information

Molecular Weight: Predicted, 64,80,75 kDa; observed, 64 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB445

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) antibody (Cat#2355). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) antibody (Cat#2355, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of H9C2 cells with Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) antibody (Cat#2355, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) expression in H9c2 cells using Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) antibody (Cat#2355, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PAK4/PAK5/PAK6
  • Catalog：
    2355
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) antibody (Cat#2355). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) antibody (Cat#2355, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of H9C2 cells with Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) antibody (Cat#2355, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) expression in H9c2 cells using Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) antibody (Cat#2355, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602).
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