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Home>Product>Primary Antibody

HK1 Mouse mAb

#6803

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Western blotting analysis using HK1 antibody (Cat#6803). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HK1 antibody (Cat#6803, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with HK1 antibody (Cat#6803, 1:1,000). Nuclei were stained blue with DAPI; HK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HK1
  • Catalog：
    6803
  • Application：
    WB, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name HK1 Mouse mAb
Aliases HK1; Hexokinase 1; Brain Form Hexokinase; Hexokinase Type I; Hexokinase-1; Hexokinase-A; EC 2.7.1.1; HKI; Neuropathy, Hereditary Motor And Sensory, Russe Type; Glycolytic Enzyme; Hexokinase IR; Hexokinase; EC 2.7.1; NEDVIBA; CNSHA5; HK1-Ta; HK1-Tb; HK1-Tc; HMSNR; HXK1; NMSR; RP79; HK I; HKD; HK
Background

Gene Name: HK1

NCBI Gene Entry: 3098

UniProt Entry: P19367

Application Information

Molecular Weight: Predicted, 102 kDa; observed, 120 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB915

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen Recombinant protein of human HK1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:4,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using HK1 antibody (Cat#6803). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HK1 antibody (Cat#6803, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HT-1080 cells with HK1 antibody (Cat#6803, 1:1,000). Nuclei were stained blue with DAPI; HK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HK1
  • Catalog：
    6803
  • Application：
    WB, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using HK1 antibody (Cat#6803). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HK1 antibody (Cat#6803, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with HK1 antibody (Cat#6803, 1:1,000). Nuclei were stained blue with DAPI; HK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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