Western blotting analysis using Bcl-2 antibody (Cat#68142). Bcl-2 expression in wild type (WT) and BCL2 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Bcl-2 antibody (Cat#68142, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using Bcl-2 antibody (Cat#68142). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Bcl-2 antibody (Cat#68142, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Bcl-2 antibody (Cat#68142). Bcl-2 expression in wild type (WT) and Bcl-2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Bcl-2 antibody (Cat#68142, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Bcl-2 expression in HepG2 cells using Bcl-2 antibody (Cat#68142, 1:2,000). Green, isotype control;red, Bcl-2.
Validation of Bcl-2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Bcl-2 antibody (Cat#68142, 1:2,000) and analyzed using BD flow cytometer.
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using bcl-2 antibody (Cat#68142, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
