KD-Validated SLBP Recombinant Rabbit mAb - Product

KD-Validated SLBP Recombinant Rabbit mAb

#65605

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Recombinant Antibody
shRNA Knockdown Validated

Western blotting analysis using SLBP antibody (Cat#65605). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SLBP antibody (Cat#65605, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using SLBP antibody (Cat#65605). SLBP expression in wild-type (WT) and SLBP shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SLBP antibody (Cat#65605, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Flow cytometric analysis of SLBP expression in HeLa cells using SLBP antibody (Cat#65605, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, SLBP.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

SLBP
Catalog：

65605
Application：

WB, FCM
Species Reactivity：

H

Information

Data

Information

Product Name	KD-Validated SLBP Recombinant Rabbit mAb
Aliases	SLBP; Stem-Loop Histone MRNA Binding Protein; HBP; Stem-Loop (Histone) Binding Protein; Histone RNA Hairpin-Binding Protein; Stem-Loop Binding Protein; Histone Binding Protein; Histone Stem-Loop Binding Protein; Histone Stem-Loop-Binding Protein; Hairpin Binding Protein, Histone
Background	Gene Name: SLBP NCBI Gene Entry: 7884 UniProt Entry: Q14493
Application Information	Molecular Weight: Predicted, 31 kDa; observed, 37 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB4485 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human SLBP
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note	This product is for research use only.

Data

Western blotting analysis using SLBP antibody (Cat#65605). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SLBP antibody (Cat#65605, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using SLBP antibody (Cat#65605). SLBP expression in wild-type (WT) and SLBP shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SLBP antibody (Cat#65605, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of SLBP expression in HeLa cells using SLBP antibody (Cat#65605, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, SLBP.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

SLBP
Catalog：

65605
Application：

WB, FCM
Species Reactivity：

H

Datasheet

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