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Home>Product>Primary Antibody

TUBB4A Recombinant Rabbit mAb

#5199

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Recombinant Antibody
Western blotting analysis using TUBB4A antibody (Cat#5199). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TUBB4A antibody (Cat#5199, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with TUBB4A antibody (Cat#5199, 1:1,000). Nuclei were stained blue with DAPI; TUBB4A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of TUBB4A expression in HAP-1 cells using TUBB4A antibody (Cat#5199, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TUBB4A.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    TUBB4A
  • Catalog：
    5199
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name TUBB4A Recombinant Rabbit mAb
Aliases TUBB4A; Tubulin Beta 4A Class IVa; Beta-5; TUBB4; Dystonia 4, Torsion (Autosomal Dominant); Tubulin Beta-4A Chain; Tubulin Beta-4 Chain; Tubulin, Beta 4; DYT4; Tubulin, Beta 4 Class IVa; Class IVa Beta-Tubulin; Tubulin, Beta, 5; Tubulin 5 Beta; TUBB5
Background

Gene Name: TUBB4A

NCBI Gene Entry: 10382

UniProt Entry: P04350

Application Information

Molecular Weight: Predicted, 50 kDa; observed, 50 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB120

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human beta IV Tubulin
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:100-1:1,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using TUBB4A antibody (Cat#5199). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TUBB4A antibody (Cat#5199, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with TUBB4A antibody (Cat#5199, 1:1,000). Nuclei were stained blue with DAPI; TUBB4A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of TUBB4A expression in HAP-1 cells using TUBB4A antibody (Cat#5199, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TUBB4A.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    TUBB4A
  • Catalog：
    5199
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using TUBB4A antibody (Cat#5199). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TUBB4A antibody (Cat#5199, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with TUBB4A antibody (Cat#5199, 1:1,000). Nuclei were stained blue with DAPI; TUBB4A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of TUBB4A expression in HAP-1 cells using TUBB4A antibody (Cat#5199, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TUBB4A.
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