Western blotting analysis using Olig3 antibody (Cat#2263). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Olig3 antibody (Cat#2263, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Olig3 expression in HAP-1 cells using Olig3 antibody (Cat#2263, 1:2,000). Green, isotype control; red, Olig3.
Immunocytochemical staining of HAP-1 cells with Olig3 antibody (Cat#2263, 1:1,000). Nuclei were stained blue with DAPI; Olig3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:High. Scale bar, 20 μm.
OLIG3; Oligodendrocyte Transcription Factor 3; BHLHe20; Bhlhb7; Class E Basic Helix-Loop-Helix Protein 20; Class B Basic Helix-Loop-Helix Protein 7; Oligo3; BHLHE20; BHLHB7; BHLHb7
Western blotting analysis using Olig3 antibody (Cat#2263). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Olig3 antibody (Cat#2263, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Olig3 expression in HAP-1 cells using Olig3 antibody (Cat#2263, 1:2,000). Green, isotype control; red, Olig3.
Immunocytochemical staining of HAP-1 cells with Olig3 antibody (Cat#2263, 1:1,000). Nuclei were stained blue with DAPI; Olig3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:High. Scale bar, 20 μm.
Western blotting analysis using Olig3 antibody (Cat#2263). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Olig3 antibody (Cat#2263, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Olig3 expression in HAP-1 cells using Olig3 antibody (Cat#2263, 1:2,000). Green, isotype control; red, Olig3.
Immunocytochemical staining of HAP-1 cells with Olig3 antibody (Cat#2263, 1:1,000). Nuclei were stained blue with DAPI; Olig3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:High. Scale bar, 20 μm.
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