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Raptor Recombinant Rabbit mAb

#2441

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Recombinant Antibody
Western blotting analysis using Raptor antibody (Cat#2441). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Raptor antibody (Cat#2441, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Raptor expression in C2C12 cells using Raptor antibody (Cat#2441, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Raptor.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    RPTOR
  • Catalog:
    2441
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name Raptor Recombinant Rabbit mAb
Aliases Regulatory Associated Protein Of MTOR Complex 1; Raptor; KIAA1303; KOG1; Mip1; Regulatory-Associated Protein Of MTOR; P150 Target Of Rapamycin (TOR)-Scaffold Protein Containing WD-Repeats; Regulatory Associated Protein Of MTOR, Complex 1; P150 Target Of Rapamycin (TOR)-Scaffold Protein; Regulatory Associated Protein Of MTOR; RAPTOR
Background

Gene Name: RPTOR

NCBI Gene Entry: 57521

UniProt Entry: Q8N122

Application Information

Molecular Weight: Predicted, 149 kDa; observed, 149 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB855

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human Raptor
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using Raptor antibody (Cat#2441). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Raptor antibody (Cat#2441, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of Raptor expression in C2C12 cells using Raptor antibody (Cat#2441, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Raptor.
Western blotting analysis using Raptor antibody (Cat#2441). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Raptor antibody (Cat#2441, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Raptor expression in C2C12 cells using Raptor antibody (Cat#2441, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Raptor.
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