Western blotting analysis using caveolin 1 antibody (Cat#6565). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with caveolin 1 antibody (Cat#6565, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HACAT cells with caveolin 1 antibody (Cat#6565, 1:1,000). Nuclei were stained blue with DAPI; Caveolin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Flow cytometric analysis of caveolin 1 expression in HaCaT cells using caveolin 1 antibody (Cat#6565, 1:2,000). Green, isotype control; red, Caveolin 1.
Western blotting analysis using caveolin 1 antibody (Cat#6565). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with caveolin 1 antibody (Cat#6565, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HACAT cells with caveolin 1 antibody (Cat#6565, 1:1,000). Nuclei were stained blue with DAPI; Caveolin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Flow cytometric analysis of caveolin 1 expression in HaCaT cells using caveolin 1 antibody (Cat#6565, 1:2,000). Green, isotype control; red, Caveolin 1.
Western blotting analysis using caveolin 1 antibody (Cat#6565). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with caveolin 1 antibody (Cat#6565, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HACAT cells with caveolin 1 antibody (Cat#6565, 1:1,000). Nuclei were stained blue with DAPI; Caveolin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Flow cytometric analysis of caveolin 1 expression in HaCaT cells using caveolin 1 antibody (Cat#6565, 1:2,000). Green, isotype control; red, Caveolin 1.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000832s ]
SELECT * FROM `goods` WHERE `code` = '6565' LIMIT 1 [ RunTime:0.002406s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.001037s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000945s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.001053s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 10309 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001501s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.001288s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 10309 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000718s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000737s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000854s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.090397s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5644,5267,7874,5316,7053,8172,6317,9744,7632,5837) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001466s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000482s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000264s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000586s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000225s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000357s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000431s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000300s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000198s ]
