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Home>Product>Primary Antibody

PIAS1+PIAS2 Recombinant Rabbit mAb

#2455

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Recombinant Antibody
Western blotting analysis using PIAS1+PIAS2 antibody (Cat#2455). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PIAS1+PIAS2 antibody (Cat#2455, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with PIAS1+PIAS2 antibody (Cat#2455, 1:1,000). Nuclei were stained blue with DAPI; PIAS1+PIAS2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PIAS1+PIAS2 expression in HeLa cells using PIAS1+PIAS2 antibody (Cat#2455, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PIAS1+PIAS2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PIAS1/ PIAS2
  • Catalog：
    2455
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name PIAS1+PIAS2 Recombinant Rabbit mAb
Aliases Protein Inhibitor Of Activated STAT 1; GBP; GU/RH-II; DDXBP1; ZMIZ3; DEAD/H (Asp-Glu-Ala-Asp/His) Box Binding Protein 1; Protein Inhibitor Of Activated STAT Protein 1; Zinc Finger, MIZ-Type Containing 3; E3 SUMO-Protein Transferase PIAS1; RNA Helicase II-Binding Protein; E3 SUMO-Protein Ligase PIAS1; DEAD/H Box-Binding Protein 1; Gu-Binding Protein; AR Interacting Protein; EC 2.3.2.-; Protein Inhibitor Of Activated STAT 2; ARIP3; ZMIZ4; Androgen Receptor-Interacting Protein 3; Protein Inhibitor Of Activated STAT X; Msx-Interacting Zinc Finger Protein; Zinc Finger, MIZ-Type Containing 4; E3 SUMO-Protein Transferase PIAS2; E3 SUMO-Protein Ligase PIAS2; DAB2-Interacting Protein; PIASX-ALPHA; PIASX-BETA; PIASX; Miz; DIP; Protein Inhibitor Of Activated STAT2; PIAS-NY Protein; EC 2.3.2.-; MIZ1; SIZ2; Miz1
Background

Gene Name: PIAS1/ PIAS2

NCBI Gene Entry: 8554 , 9063

UniProt Entry: O75925 , O75928

Application Information

Molecular Weight: Predicted, 72,68 kDa; observed, 76 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB920

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human PIAS1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using PIAS1+PIAS2 antibody (Cat#2455). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PIAS1+PIAS2 antibody (Cat#2455, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of Hela cells with PIAS1+PIAS2 antibody (Cat#2455, 1:1,000). Nuclei were stained blue with DAPI; PIAS1+PIAS2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of PIAS1+PIAS2 expression in HeLa cells using PIAS1+PIAS2 antibody (Cat#2455, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PIAS1+PIAS2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PIAS1/ PIAS2
  • Catalog：
    2455
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using PIAS1+PIAS2 antibody (Cat#2455). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PIAS1+PIAS2 antibody (Cat#2455, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with PIAS1+PIAS2 antibody (Cat#2455, 1:1,000). Nuclei were stained blue with DAPI; PIAS1+PIAS2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PIAS1+PIAS2 expression in HeLa cells using PIAS1+PIAS2 antibody (Cat#2455, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PIAS1+PIAS2.
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