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Home>Product>Primary Antibody

ATP5PB Mouse mAb

#6550

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Western blotting analysis using ATP5PB antibody (Cat#6550). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP5PB antibody (Cat#6550, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using ATP5PB antibody (Cat#6550, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with ATP5PB antibody (Cat#6550, 1:1,000). Nuclei were stained blue with DAPI; ATP5PB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium . Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ATP5PB expression in HepG2 cells using ATP5PB antibody (Cat#6550, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ATP5PB.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ATP5PB
  • Catalog：
    6550
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name ATP5PB Mouse mAb
Aliases ATP5PB; ATP Synthase Peripheral Stalk-Membrane Subunit B; ATP5F1; ATP Synthase Proton-Transporting Mitochondrial F(0) Complex Subunit B1; ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit B1; ATP Synthase, H+ Transporting, Mitochondrial Fo Complex Subunit B1; ATP Synthase Peripheral Stalk Subunit B, Mitochondrial; ATP Synthase F(0) Complex Subunit B1, Mitochondrial; ATPase Subunit B; ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit B, Isoform 1; ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit B1; ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit B; Cell Proliferation-Inducing Protein 47; ATP Synthase Subunit B, Mitochondrial; ATP Synthase B Chain, Mitochondrial; H+-ATP Synthase Subunit B; ATP Synthase Subunit B; PIG47
Background

Gene Name: ATP5PB

NCBI Gene Entry: 515

UniProt Entry: P24539

Application Information

Molecular Weight: Predicted, 29 kDa; observed, 25 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9975

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen Recombinant protein of human ATP5PB
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using ATP5PB antibody (Cat#6550). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP5PB antibody (Cat#6550, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using ATP5PB antibody (Cat#6550, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of HepG2 cells with ATP5PB antibody (Cat#6550, 1:1,000). Nuclei were stained blue with DAPI; ATP5PB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium . Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of ATP5PB expression in HepG2 cells using ATP5PB antibody (Cat#6550, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ATP5PB.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ATP5PB
  • Catalog：
    6550
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using ATP5PB antibody (Cat#6550). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP5PB antibody (Cat#6550, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using ATP5PB antibody (Cat#6550, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with ATP5PB antibody (Cat#6550, 1:1,000). Nuclei were stained blue with DAPI; ATP5PB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium . Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ATP5PB expression in HepG2 cells using ATP5PB antibody (Cat#6550, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ATP5PB.
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