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Home>Product>Primary Antibody

Solute Carrier Family 6 Member 3 Recombinant Rabbit mAb

#2770

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Recombinant Antibody
Western blotting analysis using solute carrier family 6 member 3 antibody (Cat#2770). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with solute carrier family 6 member 3 antibody (Cat#2770, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with Solute carrier family 6 member 3 antibody (Cat#2770, 1:1,000). Nuclei were stained blue with DAPI; Solute carrier family 6 member 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Solute carrier family 6 member 3 expression in HT-1080 cells using Solute carrier family 6 member 3 antibody (Cat#2770, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Solute carrier family 6 member 3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SLC6A3
  • Catalog：
    2770
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name Solute Carrier Family 6 Member 3 Recombinant Rabbit mAb
Aliases SLC6A3; Solute Carrier Family 6 Member 3; DAT; Sodium-Dependent Dopamine Transporter; DAT1; Solute Carrier Family 6 (Neurotransmitter Transporter, Dopamine), Member 3; Solute Carrier Family 6 (Neurotransmitter Transporter), Member 3; Dopamine Transporter 1; DA Transporter; Dopamine Transporter; PKDYS1; PKDYS
Background

Gene Name: SLC6A3

NCBI Gene Entry: 6531

UniProt Entry: Q01959

Application Information

Molecular Weight: Predicted, 68 kDa; observed, 40 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2160

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Dopamine Transporter
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using solute carrier family 6 member 3 antibody (Cat#2770). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with solute carrier family 6 member 3 antibody (Cat#2770, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HT-1080 cells with Solute carrier family 6 member 3 antibody (Cat#2770, 1:1,000). Nuclei were stained blue with DAPI; Solute carrier family 6 member 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Solute carrier family 6 member 3 expression in HT-1080 cells using Solute carrier family 6 member 3 antibody (Cat#2770, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Solute carrier family 6 member 3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SLC6A3
  • Catalog：
    2770
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using solute carrier family 6 member 3 antibody (Cat#2770). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with solute carrier family 6 member 3 antibody (Cat#2770, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with Solute carrier family 6 member 3 antibody (Cat#2770, 1:1,000). Nuclei were stained blue with DAPI; Solute carrier family 6 member 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Solute carrier family 6 member 3 expression in HT-1080 cells using Solute carrier family 6 member 3 antibody (Cat#2770, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Solute carrier family 6 member 3.
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