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RAS Like Proto-Oncogene A Recombinant Rabbit mAb

#2497

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Recombinant Antibody
Western blotting analysis using RALA antibody (Cat#2497). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RALA antibody (Cat#2497, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of RALA expression in HepG2 cells using RALA antibody (Cat#2497, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, RALA.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    RALA
  • Catalog:
    2497
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name RAS Like Proto-Oncogene A Recombinant Rabbit mAb
Aliases RAS Like Proto-Oncogene A; Ras-Related Protein Ral-A; RAL; V-Ral Simian Leukemia Viral Oncogene Homolog A (Ras Related); Ras Family Small GTP Binding Protein RALA; Ras Related GTP Binding Protein A; RAS-Like Protein A; RALA Ras Like Proto-Oncogene A; EC 3.6.5.2; HINCONS
Background

Gene Name: RALA

NCBI Gene Entry: 5898

UniProt Entry: P11233

Application Information

Molecular Weight: Predicted, 24 kDa; observed, 22 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1125

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human RalA
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using RALA antibody (Cat#2497). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RALA antibody (Cat#2497, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of RALA expression in HepG2 cells using RALA antibody (Cat#2497, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, RALA.
Western blotting analysis using RALA antibody (Cat#2497). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RALA antibody (Cat#2497, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of RALA expression in HepG2 cells using RALA antibody (Cat#2497, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, RALA.
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