Western blotting analysis using timeless circadian regulator antibody (Cat#4654). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with timeless circadian regulator antibody (Cat#4654, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Timeless circadian regulator expression in HepG2 cells using Timeless circadian regulator antibody (Cat#4654, 1:2,000). Green, isotype control; red, Timeless circadian regulator.
Immunocytochemical staining of HepG2 cells with Timeless circadian regulator antibody (Cat#4654, 1:1,000). Nuclei were stained blue with DAPI; Timeless circadian regulator was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using timeless circadian regulator antibody (Cat#4654). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with timeless circadian regulator antibody (Cat#4654, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Timeless circadian regulator expression in HepG2 cells using Timeless circadian regulator antibody (Cat#4654, 1:2,000). Green, isotype control; red, Timeless circadian regulator.
Immunocytochemical staining of HepG2 cells with Timeless circadian regulator antibody (Cat#4654, 1:1,000). Nuclei were stained blue with DAPI; Timeless circadian regulator was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using timeless circadian regulator antibody (Cat#4654). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with timeless circadian regulator antibody (Cat#4654, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Timeless circadian regulator expression in HepG2 cells using Timeless circadian regulator antibody (Cat#4654, 1:2,000). Green, isotype control; red, Timeless circadian regulator.
Immunocytochemical staining of HepG2 cells with Timeless circadian regulator antibody (Cat#4654, 1:1,000). Nuclei were stained blue with DAPI; Timeless circadian regulator was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001027s ]
SELECT * FROM `goods` WHERE `code` = '4654' LIMIT 1 [ RunTime:0.000667s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000553s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000552s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000512s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5964 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000466s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000632s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5964 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000460s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000544s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000381s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.080967s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (6058,343,5566,8343,5999,6220,8288,7547,8454,10512) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000892s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000441s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000208s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000462s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000224s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000307s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000386s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000222s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000206s ]
