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Home>Product>Primary Antibody

Phospho-POLR2A (S5) Recombinant Rabbit mAb

#2508

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Recombinant Antibody
Western blotting analysis using Phospho-POLR2A (S5) antibody (Cat#2508). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-POLR2A (S5) antibody (Cat#2508, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Phospho-POLR2A (S5) antibody (Cat#2508, 1:1,000). Nuclei were stained blue with DAPI; Phospho-POLR2A (S5) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-POLR2A (S5) expression in C2C12 cells using Phospho-POLR2A (S5) antibody (Cat#2508, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-POLR2A (S5).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    POLR2A
  • Catalog：
    2508
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Phospho-POLR2A (S5) Recombinant Rabbit mAb
Aliases RNA Polymerase II Subunit A; RNA Polymerase II Subunit B1; POLRA; POLR2; RPB1; DNA-Directed RNA Polymerase II Largest Subunit, RNA Polymerase II 220 Kd Subunit; Polymerase (RNA) II (DNA Directed) Polypeptide A, 220kDa; DNA-Directed RNA Polymerase III Largest Subunit; DNA-Directed RNA Polymerase II Subunit RPB1; RNA-Directed RNA Polymerase II Subunit RPB1; DNA-Directed RNA Polymerase II Subunit A; EC 2.7.7.6; Polymerase (RNA) II Subunit A; EC 2.7.7.48; HRPB220; NEDHIB; RpIILS; HsRPB1; RPBh1; RPOL2; RPO2
Background

Gene Name: POLR2A

NCBI Gene Entry: 5430

UniProt Entry: P24928

Application Information

Molecular Weight: Predicted, 217 kDa; observed, 270 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1180

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-POLR2A (S5)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Phospho-POLR2A (S5) antibody (Cat#2508). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-POLR2A (S5) antibody (Cat#2508, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with Phospho-POLR2A (S5) antibody (Cat#2508, 1:1,000). Nuclei were stained blue with DAPI; Phospho-POLR2A (S5) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Phospho-POLR2A (S5) expression in C2C12 cells using Phospho-POLR2A (S5) antibody (Cat#2508, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-POLR2A (S5).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    POLR2A
  • Catalog：
    2508
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Phospho-POLR2A (S5) antibody (Cat#2508). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-POLR2A (S5) antibody (Cat#2508, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Phospho-POLR2A (S5) antibody (Cat#2508, 1:1,000). Nuclei were stained blue with DAPI; Phospho-POLR2A (S5) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-POLR2A (S5) expression in C2C12 cells using Phospho-POLR2A (S5) antibody (Cat#2508, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-POLR2A (S5).
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