KD-Validated TIMM23 Mouse mAb - Product - Hefei GenuIN Biotech Co., Ltd.

KD-Validated TIMM23 Mouse mAb

#61908

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Knockdown Validated

Western blotting analysis using TIMM23 antibody (Cat#61908). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TIMM23 antibody (Cat#61908, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using TIMM23 antibody (Cat#61908). TIMM23 expression in wild-type (WT) and TIMM23 knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with TIMM23 antibody (Cat#61908, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Flow cytometric analysis of TIMM23 expression in HeLa cells using TIMM23 antibody (Cat#61908, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TIMM23.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

TIMM23
Catalog：

61908
Application：

WB, FCM
Species Reactivity：

H, M, R

Information

Data

Information

Product Name	KD-Validated TIMM23 Mouse mAb
Aliases	TIMM23; Translocase Of Inner Mitochondrial Membrane 23; TIM23; Mitochondrial Import Inner Membrane Translocase Subunit Tim23; Translocase Of Inner Mitochondrial Membrane 23 Homolog (Yeast); Translocase Of Inner Mitochondrial Membrane 23 Homolog; Translocase Of The Inner Mitochondrial Membrane
Background	Gene Name: TIMM23 NCBI Gene Entry: 100287932 UniProt Entry: O14925
Application Information	Molecular Weight: Predicted, 22 kDa; observed, 20 kDa Clonality: Mouse monoclonal antibody Clone ID: 25GB4145 Species Reactivity: Human,mouse,rat Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	Recombinant protein of human TIMM23
Isotype	Mouse IgG2b
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:200-1:2,000
Note	This product is for research use only.

Data

Western blotting analysis using TIMM23 antibody (Cat#61908). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TIMM23 antibody (Cat#61908, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using TIMM23 antibody (Cat#61908). TIMM23 expression in wild-type (WT) and TIMM23 knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with TIMM23 antibody (Cat#61908, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of TIMM23 expression in HeLa cells using TIMM23 antibody (Cat#61908, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TIMM23.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

TIMM23
Catalog：

61908
Application：

WB, FCM
Species Reactivity：

H, M, R

Datasheet

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