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Home>Product>Primary Antibody

Sphingosine-1-Phosphate Receptor 1 Recombinant Rabbit mAb

#2567

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Recombinant Antibody
Western blotting analysis using Sphingosine-1-phosphate receptor 1 antibody (Cat#2567). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Sphingosine-1-phosphate receptor 1 antibody (Cat#2567, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Sphingosine-1-phosphate receptor 1 antibody (Cat#2567, 1:1,000). Nuclei were stained blue with DAPI; Sphingosine-1-phosphate receptor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Sphingosine-1-phosphate receptor 1 expression in HepG2 cells using Sphingosine-1-phosphate receptor 1 antibody (Cat#2567, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Sphingosine-1-phosphate receptor 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    S1PR1
  • Catalog：
    2567
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Sphingosine-1-Phosphate Receptor 1 Recombinant Rabbit mAb
Aliases S1PR1; Sphingosine-1-Phosphate Receptor 1; D1S3362; Edg-1; CD363; EDG1; Endothelial Differentiation, Sphingolipid G-Protein-Coupled Receptor, 1;Endothelial Differentiation G-Protein Coupled Receptor 1 ; Sphingosine 1-Phosphate Receptor Edg-1; Sphingosine 1-Phosphate Receptor 1; S1P Receptor Edg-1; S1P Receptor 1; CHEDG1; S1P1; Sphingosine 1-Phosphate Receptor EDG1; CD363 Antigen; ECGF1
Background

Gene Name: S1PR1

NCBI Gene Entry: 1901

UniProt Entry: P21453

Application Information

Molecular Weight: Predicted, 43 kDa; observed, 43 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1435

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human EDG1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Sphingosine-1-phosphate receptor 1 antibody (Cat#2567). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Sphingosine-1-phosphate receptor 1 antibody (Cat#2567, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with Sphingosine-1-phosphate receptor 1 antibody (Cat#2567, 1:1,000). Nuclei were stained blue with DAPI; Sphingosine-1-phosphate receptor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Sphingosine-1-phosphate receptor 1 expression in HepG2 cells using Sphingosine-1-phosphate receptor 1 antibody (Cat#2567, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Sphingosine-1-phosphate receptor 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    S1PR1
  • Catalog：
    2567
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Sphingosine-1-phosphate receptor 1 antibody (Cat#2567). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Sphingosine-1-phosphate receptor 1 antibody (Cat#2567, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Sphingosine-1-phosphate receptor 1 antibody (Cat#2567, 1:1,000). Nuclei were stained blue with DAPI; Sphingosine-1-phosphate receptor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Sphingosine-1-phosphate receptor 1 expression in HepG2 cells using Sphingosine-1-phosphate receptor 1 antibody (Cat#2567, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Sphingosine-1-phosphate receptor 1.
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