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Sarcoglycan Delta Recombinant Rabbit mAb

#2573

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Recombinant Antibody
Western blotting analysis using Sarcoglycan delta antibody (Cat#2573). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Sarcoglycan delta antibody (Cat#2573, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Sarcoglycan delta antibody (Cat#2573, 1:1,000). Nuclei were stained blue with DAPI; Sarcoglycan delta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    SGCD
  • Catalog:
    2573
  • Application:
    WB, ICC
  • Species Reactivity:
    H, M
Information
Product Name Sarcoglycan Delta Recombinant Rabbit mAb
Aliases SGCD; Sarcoglycan Delta; CMD1L; DAGD; Sarcoglycan, Delta (35kDa Dystrophin-Associated Glycoprotein); 35 KDa Dystrophin-Associated Glycoprotein; Delta-Sarcoglycan;
Delta-SG; LGMD2F; 35DAG; Dystrophin Associated Glycoprotein, Delta Sarcoglycan; Placental Delta Sarcoglycan; SG-Delta; LGMDR6; SGCDP; SGD
Background

Gene Name: SGCD

NCBI Gene Entry: 6444

UniProt Entry: Q92629

Application Information

Molecular Weight: Predicted, 32 kDa; observed, 35 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1465

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human delta Sarcoglycan
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Sarcoglycan delta antibody (Cat#2573). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Sarcoglycan delta antibody (Cat#2573, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with Sarcoglycan delta antibody (Cat#2573, 1:1,000). Nuclei were stained blue with DAPI; Sarcoglycan delta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Western blotting analysis using Sarcoglycan delta antibody (Cat#2573). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Sarcoglycan delta antibody (Cat#2573, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Sarcoglycan delta antibody (Cat#2573, 1:1,000). Nuclei were stained blue with DAPI; Sarcoglycan delta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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