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CDC73 Mouse mAb

#6438

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Western blotting analysis using CDC73 antibody (Cat#6438). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CDC73 antibody (Cat#6438, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CDC73 expression in HAP-1 cells using CDC73 antibody (Cat#6438, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CDC73.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    CDC73
  • Catalog:
    6438
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name CDC73 Mouse mAb
Aliases CDC73; Cell Division Cycle 73; Parafibromin; C1orf28; HRPT2; FIHP; Paf1/RNA Polymerase II Complex Component; Cell Division Cycle Protein 73 Homolog; Familial Isolated Hyperparathyroidism; Hyperparathyroidism 2 Protein; HRPT1; Cell Division Cycle 73, Paf1/RNA Polymerase II Complex Component, Homolog (S. Cerevisiae); Cell Division Cycle 73 Paf1/RNA Polymerase II Complex Component-Like Protein; Cell Division Cycle 73, Paf1/RNA Polymerase II Complex Component, Homolog; Hyperparathyroidism 2 (With Jaw Tumor); Chromosome 1 Open Reading Frame 28; Hyperparathyroidism 1; HPTJT; HYX
Background

Gene Name: CDC73

NCBI Gene Entry: 79577

UniProt Entry: Q6P1J9

Application Information

Molecular Weight: Predicted, 61 kDa; observed, 60 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9095

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human CDC73
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using CDC73 antibody (Cat#6438). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CDC73 antibody (Cat#6438, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of CDC73 expression in HAP-1 cells using CDC73 antibody (Cat#6438, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CDC73.
Western blotting analysis using CDC73 antibody (Cat#6438). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CDC73 antibody (Cat#6438, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CDC73 expression in HAP-1 cells using CDC73 antibody (Cat#6438, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CDC73.
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