Western blotting analysis using Janus kinase 1 antibody (Cat#61215). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Janus kinase 1 antibody (Cat#61215, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using janus kinase 1 antibody (Cat#61215). Janus kinase 1 expression in wild type (WT) and janus kinase 1 (JAK1) shRNA knockdown (KD) 293T cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with janus kinase 1 antibody (Cat#61215, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Janus kinase 1 antibody (Cat#61215, 1:1,000). Nuclei were stained blue with DAPI; Janus kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Janus kinase 1 expression in HepG2 cells using Janus kinase 1 antibody (Cat#61215, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Janus kinase 1.
