Western blotting analysis using C17orf58 antibody (Cat#3823). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with C17orf58 antibody (Cat#3823, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of C17orf58 expression in HT-1080 cells using C17orf58 antibody (Cat#3823, 1:1,000). Green, isotype control; red, C17orf58.
Immunocytochemical staining of HT-1080 cells with C17orf58 antibody (Cat#3823, 1:1,000). Nuclei were stained blue with DAPI; C17orf58 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using C17orf58 antibody (Cat#3823). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with C17orf58 antibody (Cat#3823, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of C17orf58 expression in HT-1080 cells using C17orf58 antibody (Cat#3823, 1:1,000). Green, isotype control; red, C17orf58.
Immunocytochemical staining of HT-1080 cells with C17orf58 antibody (Cat#3823, 1:1,000). Nuclei were stained blue with DAPI; C17orf58 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using C17orf58 antibody (Cat#3823). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with C17orf58 antibody (Cat#3823, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of C17orf58 expression in HT-1080 cells using C17orf58 antibody (Cat#3823, 1:1,000). Green, isotype control; red, C17orf58.
Immunocytochemical staining of HT-1080 cells with C17orf58 antibody (Cat#3823, 1:1,000). Nuclei were stained blue with DAPI; C17orf58 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.002922s ]
SELECT * FROM `goods` WHERE `code` = '3823' LIMIT 1 [ RunTime:0.004153s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.001733s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.003521s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.001241s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5208 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.005396s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.002429s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5208 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.003388s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.005825s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.002076s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.197990s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (6160,4357,5382,7123,5550,5793,7328,7583,4014,5764) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000840s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000483s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000221s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000506s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000264s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000313s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000451s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000225s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000352s ]
