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Home>Product>Primary Antibody

KD-Validated UBE2T Recombinant Rabbit mAb

#62664

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using UBE2T antibody (Cat#62664). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBE2T antibody (Cat#62664, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using UBE2T antibody (Cat#62664). UBE2T expression in wild type (WT) and UBE2T shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with UBE2T antibody (Cat#62664, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with UBE2T antibody(Cat#62664, 1:1,000). Nuclei were stained blue with DAPI; UBE2T was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HepG2 cells with UBE2T antibody(Cat#62664, 1:1,000). Nuclei were stained blue with DAPI; UBE2T was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of UBE2T expression in HAP-1 cells using UBE2T antibody (Cat#62664, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, UBE2T.
Validation of UBE2T knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with UBE2T antibody (Cat#62664, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
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  • Gene Symbol：
    UBE2T
  • Catalog：
    62664
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated UBE2T Recombinant Rabbit mAb
Aliases UBE2T; Ubiquitin Conjugating Enzyme E2 T; HSPC150; FANCT; Cell Proliferation-Inducing Gene 50 Protein; Ubiquitin-Conjugating Enzyme E2 T; E2 Ubiquitin-Conjugating Enzyme T; Ubiquitin Carrier Protein T; Ubiquitin-Protein Ligase T; HSPC150 Protein Similar To Ubiquitin-Conjugating Enzyme; Ubiquitin-Conjugating Enzyme E2T (Putative); Ubiquitin-Conjugating Enzyme E2T; Ubiquitin Conjugating Enzyme E2T; EC 2.3.2.23; PIG50
Background

Gene Name: UBE2T

NCBI Gene Entry: 29089

UniProt Entry: Q9NPD8

Application Information

Molecular Weight: Predicted, 23 kDa; observed, 23 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2350

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human HSPC150
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using UBE2T antibody (Cat#62664). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBE2T antibody (Cat#62664, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using UBE2T antibody (Cat#62664). UBE2T expression in wild type (WT) and UBE2T shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with UBE2T antibody (Cat#62664, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with UBE2T antibody(Cat#62664, 1:1,000). Nuclei were stained blue with DAPI; UBE2T was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Immunocytochemical staining of HepG2 cells with UBE2T antibody(Cat#62664, 1:1,000). Nuclei were stained blue with DAPI; UBE2T was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of UBE2T expression in HAP-1 cells using UBE2T antibody (Cat#62664, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, UBE2T.
  • Validation of UBE2T knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with UBE2T antibody (Cat#62664, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    UBE2T
  • Catalog：
    62664
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using UBE2T antibody (Cat#62664). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBE2T antibody (Cat#62664, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using UBE2T antibody (Cat#62664). UBE2T expression in wild type (WT) and UBE2T shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with UBE2T antibody (Cat#62664, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with UBE2T antibody(Cat#62664, 1:1,000). Nuclei were stained blue with DAPI; UBE2T was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HepG2 cells with UBE2T antibody(Cat#62664, 1:1,000). Nuclei were stained blue with DAPI; UBE2T was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of UBE2T expression in HAP-1 cells using UBE2T antibody (Cat#62664, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, UBE2T.
Validation of UBE2T knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with UBE2T antibody (Cat#62664, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
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