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Home>Product>Primary Antibody

STUB1 Recombinant Rabbit mAb

#4553

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Recombinant Antibody
Western blotting analysis using STUB1 antibody (Cat#4553). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STUB1 antibody (Cat#4553, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with STUB1 antibody (Cat#4553, 1:1,000). Nuclei were stained blue with DAPI; STUB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of STUB1 expression in C2C12 cells using STUB1 antibody (Cat#4553, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, STUB1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    STUB1
  • Catalog：
    4553
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name STUB1 Recombinant Rabbit mAb
Aliases STUB1; STIP1 Homology And U-Box Containing Protein 1; CHIP; SDCCAG7; HSPABP2; NY-CO-7; UBOX1; Carboxy Terminus Of Hsp70-Interacting Protein ; RING-Type E3 Ubiquitin; Transferase CHIP; E3 Ubiquitin-Protein Ligase CHIP; CLL-Associated Antigen KW-8; Antigen NY-CO-7; STIP1 Homology And U-Box Containing Protein 1, E3 Ubiquitin Protein Ligase; Heat Shock Protein A Binding Protein 2 (C-Terminal); STIP1 Homology And U Box-Containing Protein 1; Serologically Defined Colon Cancer Antigen 7; EC 2.3.2.27; SCAR16; SCA48
Background

Gene Name: STUB1

NCBI Gene Entry: 10273

UniProt Entry: Q9UNE7

Application Information

Molecular Weight: Predicted, 35 kDa; observed, 35 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB11790

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human STUB1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using STUB1 antibody (Cat#4553). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STUB1 antibody (Cat#4553, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of C2C12 cells with STUB1 antibody (Cat#4553, 1:1,000). Nuclei were stained blue with DAPI; STUB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of STUB1 expression in C2C12 cells using STUB1 antibody (Cat#4553, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, STUB1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    STUB1
  • Catalog：
    4553
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using STUB1 antibody (Cat#4553). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STUB1 antibody (Cat#4553, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with STUB1 antibody (Cat#4553, 1:1,000). Nuclei were stained blue with DAPI; STUB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of STUB1 expression in C2C12 cells using STUB1 antibody (Cat#4553, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, STUB1.
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