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KD-Validated ATG7 Mouse mAb

#62134

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shRNA Knockdown Validated
Western blotting analysis using ATG7 antibody (Cat#62134). ATG7 expression in wild-type (WT) and ATG7 shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATG7 antibody (Cat#62134, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    ATG7
  • Catalog:
    62134
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KD-Validated ATG7 Mouse mAb
Aliases ATG7; Autophagy Related 7; Ubiquitin-Activating Enzyme E1-Like Protein; APG7L; GSA7; Ubiquitin-Like Modifier-Activating Enzyme ATG7; ATG12-Activating Enzyme E1 ATG7; DKFZp434N0735; HAGP7; ATG7 Autophagy Related 7 Homolog (S. Cerevisiae); APG7 Autophagy 7-Like (S. Cerevisiae); Autophagy-Related Protein 7; APG7 Autophagy 7-Like; APG7-LIKE; APG7-Like; SCAR31
Background

Gene Name: ATG7

NCBI Gene Entry: 10533

UniProt Entry: O95352

Application Information

Molecular Weight: Predicted, 78 kDa; observed, 70 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB1525

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen Recombinant protein of human ATG7
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using ATG7 antibody (Cat#62134). ATG7 expression in wild-type (WT) and ATG7 shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATG7 antibody (Cat#62134, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ATG7 antibody (Cat#62134). ATG7 expression in wild-type (WT) and ATG7 shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATG7 antibody (Cat#62134, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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