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Home>Product>Primary Antibody

Cytochrome P450 2C9 Recombinant Rabbit mAb

#2077

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Recombinant Antibody
Western blotting analysis using Cytochrome P450 2C9 antibody (Cat#2077). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Cytochrome P450 2C9 antibody (Cat#2077, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Cytochrome P450 2C9 antibody (Cat#2077, 1:1,000). Nuclei were stained blue with DAPI; Cytochrome P450 2C9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Cytochrome P450 2C9 expression in HepG2 cells using Cytochrome P450 2C9 antibody (Cat#2077, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Cytochrome P450 2C9.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CYP2C9
  • Catalog：
    2077
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name Cytochrome P450 2C9 Recombinant Rabbit mAb
Aliases CYP2C9; Cytochrome P450 Family 2 Subfamily C Member 9; P450IIC9; CYP2C10; Cytochrome P450, Family 2, Subfamily C, Polypeptide 9; Cytochrome P450 PB-1; Cytochrome P450 2C9; Cytochrome P-450MP; EC 1.14.14.1; CYPIIC9; Cytochrome P450, Subfamily IIC (Mephenytoin 4-Hydroxylase), Polypeptide 9; Cytochrome P-450 S-Mephenytoin 4-Hydroxylase; Flavoprotein-Linked Monooxygenase; (R)-Limonene 6-Monooxygenase; (S)-Limonene 6-Monooxygenase; (S)-Limonene 7-Monooxygenase; S-Mephenytoin 4-Hydroxylase; Cholesterol 25-Hydroxylase; Microsomal Monooxygenase; Xenobiotic Monooxygenase; Cytochrome P450 MP-4; Cytochrome P450 MP-8; EC 1.14.14.53; EC 1.14.14.51; EC 1.14.14.52; P450-2C9; CYP2C; CPC9
Background

Gene Name: CYP2C9

NCBI Gene Entry: 1559

UniProt Entry: P11712

Application Information

Molecular Weight: Predicted, 56 kDa; observed, 50 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB5885

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Cytochrome P450 2C9
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Cytochrome P450 2C9 antibody (Cat#2077). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Cytochrome P450 2C9 antibody (Cat#2077, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with Cytochrome P450 2C9 antibody (Cat#2077, 1:1,000). Nuclei were stained blue with DAPI; Cytochrome P450 2C9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Cytochrome P450 2C9 expression in HepG2 cells using Cytochrome P450 2C9 antibody (Cat#2077, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Cytochrome P450 2C9.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CYP2C9
  • Catalog：
    2077
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using Cytochrome P450 2C9 antibody (Cat#2077). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Cytochrome P450 2C9 antibody (Cat#2077, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Cytochrome P450 2C9 antibody (Cat#2077, 1:1,000). Nuclei were stained blue with DAPI; Cytochrome P450 2C9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Cytochrome P450 2C9 expression in HepG2 cells using Cytochrome P450 2C9 antibody (Cat#2077, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Cytochrome P450 2C9.
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