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  • Primary Antibody
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Home>Product>Primary Antibody

Cadherin 1 Recombinant Rabbit mAb

#H1139

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Western blotting analysis using cadherin 1 antibody (Cat#H1139). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cadherin 1 antibody (Cat#H1139, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Immunocytochemical staining shows positive staining in paraffin-embedded rat kidney. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded mouse colon. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human hepatocellular carcinoma. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human endometrial cancer. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human colon cancer. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human breast cancer. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human stomach. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human kidney. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using cadherin 1 antibody (Cat#H1139, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining shows positive staining in paraffin-embedded human liver. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Flow cytometric analysis of Cadherin 1 expression in A549 cells using Cadherin 1 antibody (Cat#H1139, 1:2,000). Green, isotype control; red, Cadherin 1.
Please select the specifications of the product.
Specification：
20 μL，100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    CDH1
  • Catalog：
    H1139
  • Application：
    WB, IHC-P, FCM
  • Species Reactivity：
    H

Information

Data

Information
Product Name Cadherin 1 Recombinant Rabbit mAb
Aliases CDH1; Cadherin 1; Uvomorulin; CD324; UVO; Cadherin 1, Type 1, E-Cadherin (Epithelial); Epithelial Cadherin; E-Cadherin; Cadherin-1; CAM 120/80; CDHE; Calcium-Dependent Adhesion Protein, Epithelial; Epididymis Secretory Sperm Binding Protein; Cadherin 1, E-Cadherin (Epithelial); Cell-CAM 120/80; CD324 Antigen; E-Cadherin 1; UVOMORULIN; Arc-1; BCDS1; ECAD; LCAM
Background

Gene Name: CDH1

NCBI Gene Entry: 999

UniProt Entry: P12830

Application Information

Molecular Weight: Predicted, 97 kDa, Observed, 140 kDa

Clonality: Recombinant Monoclonal Antibody

Clone ID: SDT-438-45

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P), flow cytometry (FCM)
Immunogen Recombinant protein of human E-Cadherin
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 40% glycerol, 0.05% BSA and 0.03% Proclin 300.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Immunohistochemistry-Paraffin (IHC-P): 1:1,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using cadherin 1 antibody (Cat#H1139). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cadherin 1 antibody (Cat#H1139, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining shows positive staining in paraffin-embedded rat kidney. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded mouse colon. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human hepatocellular carcinoma. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human endometrial cancer. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human colon cancer. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human breast cancer. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human stomach. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human kidney. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using cadherin 1 antibody (Cat#H1139, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human liver. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Flow cytometric analysis of Cadherin 1 expression in A549 cells using Cadherin 1 antibody (Cat#H1139, 1:2,000). Green, isotype control; red, Cadherin 1.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    CDH1
  • Catalog：
    H1139
  • Application：
    WB, IHC-P, FCM
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using cadherin 1 antibody (Cat#H1139). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cadherin 1 antibody (Cat#H1139, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Immunocytochemical staining shows positive staining in paraffin-embedded rat kidney. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded mouse colon. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human hepatocellular carcinoma. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human endometrial cancer. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human colon cancer. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human breast cancer. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human stomach. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human kidney. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using cadherin 1 antibody (Cat#H1139, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining shows positive staining in paraffin-embedded human liver. E-Cadherin antibody (Cat#H1139) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Flow cytometric analysis of Cadherin 1 expression in A549 cells using Cadherin 1 antibody (Cat#H1139, 1:2,000). Green, isotype control; red, Cadherin 1.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
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  1. CONNECT:[ UseTime:0.000510s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000829s ]
  3. SELECT * FROM `goods` WHERE `code` = 'H1139' LIMIT 1 [ RunTime:0.000469s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000467s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000378s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000388s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 29 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000776s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000428s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 29 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000277s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000344s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000259s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.052276s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (8808,7207,4947,9583,7047,6819,5831,6065,9007,4170) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000728s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000473s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000213s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000540s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000251s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000289s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000365s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000325s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000293s ]
0.092205s