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Home>Product>Primary Antibody

KD-Validated SMARCD1 Recombinant Rabbit mAb

#65659

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using SMARCD1 antibody (Cat#65659). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMARCD1 antibody (Cat#65659, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using SMARCD1 antibody (Cat#65659). SMARCD1 expression in wild-type (WT) and SMARCD1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SMARCD1 antibody (Cat#65659, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded mouse testis tissue using SMARCD1 antibody (Cat#65659, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HeLa cells using SMARCD1 antibody (Cat#65659, 1:1,000), Top panel: wild-type (WT); Bottom panal: SMARCD1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; SMARCD1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SMARCD1 expression in HepG2 cells using SMARCD1 antibody (Cat#65659, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SMARCD1.
Validation of SMARCD1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with SMARCD1 antibody (Cat#65659, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
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  • Gene Symbol：
    SMARCD1
  • Catalog：
    65659
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated SMARCD1 Recombinant Rabbit mAb
Aliases SMARCD1; SWI/SNF Related BAF Chromatin Remodeling Complex Subunit D1; BAF60A; SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily D, Member 1; CRACD1; Rsc6p; SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily D Member 1; 60 KDa BRG-1/Brm-Associated Factor Subunit A; BRG1-Associated Factor 60A; Mammalian Chromatin Remodeling Complex BRG1-Associated Factor 60A; Chromatin Remodeling Complex BAF60A Subunit; SWI/SNF Complex 60 KDa Subunit A; SWI/SNF Complex 60 KDa Subunit; Swp73-Like Protein; CSS11
Background

Gene Name: SMARCD1

NCBI Gene Entry: 6602

UniProt Entry: Q96GM5

Application Information

Molecular Weight: Predicted, 58 kDa; observed, 58 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4745

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human SMARCD1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using SMARCD1 antibody (Cat#65659). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMARCD1 antibody (Cat#65659, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using SMARCD1 antibody (Cat#65659). SMARCD1 expression in wild-type (WT) and SMARCD1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SMARCD1 antibody (Cat#65659, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded mouse testis tissue using SMARCD1 antibody (Cat#65659, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of HeLa cells using SMARCD1 antibody (Cat#65659, 1:1,000), Top panel: wild-type (WT); Bottom panal: SMARCD1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; SMARCD1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of SMARCD1 expression in HepG2 cells using SMARCD1 antibody (Cat#65659, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SMARCD1.
  • Validation of SMARCD1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with SMARCD1 antibody (Cat#65659, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SMARCD1
  • Catalog：
    65659
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using SMARCD1 antibody (Cat#65659). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMARCD1 antibody (Cat#65659, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using SMARCD1 antibody (Cat#65659). SMARCD1 expression in wild-type (WT) and SMARCD1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SMARCD1 antibody (Cat#65659, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded mouse testis tissue using SMARCD1 antibody (Cat#65659, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HeLa cells using SMARCD1 antibody (Cat#65659, 1:1,000), Top panel: wild-type (WT); Bottom panal: SMARCD1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; SMARCD1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SMARCD1 expression in HepG2 cells using SMARCD1 antibody (Cat#65659, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SMARCD1.
Validation of SMARCD1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with SMARCD1 antibody (Cat#65659, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
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