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Home>Product>Primary Antibody

Vav1/2/3 Recombinant Rabbit mAb

#2681

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Recombinant Antibody
Western blotting analysis using Vav1/2/3 antibody (Cat#2681). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Vav1/2/3 antibody (Cat#2681, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Vav1/2/3 antibody (Cat#2681, 1:1,000). Nuclei were stained blue with DAPI; Vav1/2/3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    VAV1/VAV2/ VAV3
  • Catalog：
    2681
  • Application：
    WB, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name Vav1/2/3 Recombinant Rabbit mAb
Aliases VAV1; Vav Guanine Nucleotide Exchange Factor 1; VAV; Vav 1 Guanine Nucleotide Exchange Factor; Proto-Oncogene Vav; Vav 1 Oncogene; VAV2; Vav Guanine Nucleotide Exchange Factor 2; Guanine Nucleotide Exchange Factor VAV2; Vav 2 Oncogene; VAV-2; Vav 2 Guanine Nucleotide Exchange Factor; VAV3; Vav Guanine Nucleotide Exchange Factor 3; Vav 3 Guanine Nucleotide Exchange Factor; Guanine Nucleotide Exchange Factor VAV3; Vav 3 Oncogene; VAV-3
Background

Gene Name: VAV1/VAV2/ VAV3

NCBI Gene Entry: 7409 , 7410 , 10451

UniProt Entry: P15498 , P52735 , Q9UKW4

Application Information

Molecular Weight: Predicted, 98 kDa; observed, 98 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2645

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Vav1/2/3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Vav1/2/3 antibody (Cat#2681). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Vav1/2/3 antibody (Cat#2681, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with Vav1/2/3 antibody (Cat#2681, 1:1,000). Nuclei were stained blue with DAPI; Vav1/2/3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    VAV1/VAV2/ VAV3
  • Catalog：
    2681
  • Application：
    WB, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using Vav1/2/3 antibody (Cat#2681). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Vav1/2/3 antibody (Cat#2681, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Vav1/2/3 antibody (Cat#2681, 1:1,000). Nuclei were stained blue with DAPI; Vav1/2/3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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